Applications:
The Atlas Antifade CFM is a new version of the Atlas Antifade reagent that is optimized for confocal fluorescence microscopy protecting dyes against photobleaching (Figure 1). The glycerol-containing solution is ready-to-use and recommended for most applications. Samples mounted using Atlas Antifade CFM can be stored for one week at +4ºC in dark.

Description:
When exposed to excitation light, all fluorescent dyes fade (photobleach). The photobleaching depends on intensity- and the duration of illumination. The photon output of a dye represents the average number of cycles of excitation followed by fluorescence emission that the dye undergoes before it is irreversibly photobleached.

The Atlas Antifade CFM minimizes photobleaching of experimental samples. It allows the visualization of fluorophores across entire spectrum: DAPI, Cy2, Alexa 488, FITC, R6G, Cy3, Rhodamine, Alexa 555, Cy3.5, TexasRed, Cy5, Cy5.5 and Cy7.

Atlas Antifade CFM offers the same or higher quality results at a lower cost level compared to alternative products.

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Figure 1. HeLa cells in whole fluorescence (left) and DIC (right), image fixed with 3% paraformaldehyd in PBS solution, mounted with Atlas Antifade CFM and using Alexa Fluor 488.

Safety warnings and precautions:
Atlas Antifade CFM contains 2.5 mM azide. This product should be handled only by persons trained in laboratory techniques and used in accordance with the principles of good laboratory practice. All chemicals should be considered potentially hazardous: therefore, when handling chemical reagents, it is advisable that suitable protective clothing, such as laboratory overalls and gloves be worn. In case of contact with skin or eyes, wash immediately with water.

WARNING: For research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.

Storage conditions: -20ºC *DO NOT FREEZE AT LOWER TEMPERATURES*

To place an order, please fill in our order form and email to info@bioatlas.com or fax +372 616 6755.