Applications:
- Polymerase chain reaction setup at room temperature.
- Effective incorporation of modified nucleotides.

Source: Recombinant E. coli strain with cloned gene encoding Thermus aquaticus DNA polymerase.

Description: Atlas HotTaq DNA Polymerase catalyzes 5´ to 3´synthesis of DNA. The enzyme has been proved not having the 3´ to 5´ exonuclease activity. Prior the first PCR step the Atlas HotTaq DNA Polymerase should be activated by 15 minute incubation at 95-97°C.

Unit definition: One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.

Quality control: Free of detectable, non-specific nucleases. Activity and stability tested at 20, 30 and 40 cycles of PCR reaction at 95°C. Tested for the absence of human DNA contamination by PCR with Alu-specific primers.

Storage conditions: 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol. Store at -20°C. Guaranteed stable for 12 months when properly stored.

Concentration: 10 units/µl

Supplied with:
Atlas 10x Taq Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl,
0.8% Nonident P40.
Atlas 10x Taq Buffer with (NH₄)₂SO₄: 750 mM Tris-HCl (pH 8.8 at 25°C),
200 mM (NH₄)₂SO₄, 0.1% Tween 20.
Atlas 25 mM MgCl₂

Comments: This product is supplied for research use only.

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