Applications:
- Easy visualization of enzyme addition.
- Visualization of complete reaction mixing.
- Direct loading of samples following amplification.

Source: Recombinant E. coli strain with cloned gene encoding Thermus aquaticus DNA polymerase.

Description: Atlas RedTaq DNA Polymerase catalyzes 5´ to 3´synthesis of DNA. The enzyme has been proved not having the 3´ to 5´exonuclease activity. The enzyme has proven to have high amplification yield, be stable at high temperature. Added inert dye will not have any interference to the reaction. Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred. Samples can be loaded directly onto an agarose gel for electrophoresis with no loading dye addition.

Unit definition: One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.

Quality control: Free of detectable, non-specific nucleases.

Storage conditions: 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol. Store at -20°C. Guaranteed stable for 6 months when properly stored.

Concentration: 1 unit/µl

Supplied with:
Atlas 10x Taq Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% Nonident P40.
Atlas 10x Taq Buffer with (NH₄)₂SO₄: 750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM (NH₄)₂SO₄, 0.1% Tween 20.
Atlas 25 mM MgCl₂

Comments: This product is supplied for research use only.

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To place an order, please fill in our order form, and email to info@bioatlas.com or fax +372 616 6755.