Applications:
- Polymerase chain reaction (PCR).
- Automated sequencing reactions.

Source: Recombinant E. coli strain with cloned gene encoding Thermus aquaticus DNA polymerase.

Description: Atlas Taq DNA Polymerase (Figure 1) catalyzes 5´ to 3´ synthesis of DNA. The enzyme has been proved not having the 3´ to 5´ exonuclease activity. The enzyme has proven to have high amplification yield, be stable at high temperature.

Figure 1. Amplification of 420 bp target DNA using Atlas Taq DNA Polymerase.
M – Atlas Star 100 bp DNA Ladder
1-3 – Atlas Taq DNA Polymerase

Unit definition: One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.

Quality control: Free of detectable, non-specific nucleases.

Storage conditions: 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol. Store at -20°C. Guaranteed stable for 12 months when properly stored.

Concentration: 5 units/µl

Comments: This product is supplied for research use only.

Related products:

Product	Quantity	Cat. #
Atlas Taq 2x PCR Mix	100 rxn	BA01501
Atlas 10x Taq Buffer	1.8 mL	BA00151
Atlas 10x Taq Buffer with (NH4)2SO4	1.8 mL	BA00152
Atlas 25 mM MgCl2	1.8 mL	BA00153
Atlas 25 mM MgCl2	5 x 1.8 mL	BA00154
Atlas PCR Water	10 x 1.8 mL	BA01201
Atlas PCR Water	25 x 1.8 mL	BA01202
Atlas PCR Water	5 x 10 mL	BA01203
Atlas 10 mM Nucleotide Mix	1 mL	BA01602
Atlas 2 mM Nucleotide Mix	1 mL	BA01601