Description: Ready-mixed phosphate buffered saline powder to be dissolved in 1 L of distilled water. On dilution, the resultant 1x PBS will have final concentration: 137 mM Sodium Chloride, 10 mM Phosphate, 2.7 mM Potassium Chloride, pH 7.4.

Storage conditions: Store at room temperature.
 

Comments: This product is for research use only.

Description: Ready-mixed SSC powder to be dissolved in 1 L of distilled water. On dilution, the resultant 1x SSC will have final concentration: 150 mM Sodium Chloride, 15 mM Sodium Citrate, pH 7.0.

Storage conditions: Store at room temperature.
 

Comments: This product is for research use only.

Description: Ready-mixed Tris-Borate-EDTA Buffer powder to be dissolved in 1 L of distilled water. On dilution, the resultant 1x TBE solution will have final concentration: 89 mM Tris Base, 89 mM Borate, 2 mM EDTA, pH 8.2.

Storage conditions: Store at room temperature. The reconstituted buffer is stable for several days at 4°C if 0.2 µm filtered.

Comments: These products are for research use only.

Description: Ready-mixed Tris-Glycine Buffer powder to be dissolved in 1 L of distilled water. On dilution, the resultant 1x Tris-Glycine Buffer will have final concentration: 25 mM Tris, 192 mM glycine, pH 8.3. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose. The 10x Tris-glycine buffer is diluted to 1x with methanol and water to make a solution containing 20% methanol.

Storage conditions: Store at room temperature.

Comments: This product is for research use only.

Description: Ready-mixed Tris-Glycine-SDS Buffer powder to be dissolved in 1 L of distilled water. On dilution, the resultant 1x Tris-Glycine-SDS Buffer will have final concentration: 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.6. Tris-glycine-SDS Buffer is the most commonly used buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. It is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

Storage conditions: Store at room temperature.

Comments: This product is for research use only.