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Applications:
- Hot Start PCR
- DHPLC
- TA cloning.
Description: Titan HotTaq 10x PCR Mix is a ready-to-use solution containing all reagents required for Hot Start PCR (except template, primers and water). According to the type of the reaction the selection of provided 10x Reaction Buffers B1 or B2 (described below) should cover the needs to perform the reaction.
Composition:
Titan HotTaq 10x PCR Mix
- Titan HotTaq DNA polymerase
- 10x Reaction Buffer B1(Tris-HCl and (NH4)2SO4) or 10x Reaction Buffer B2 (Tris-HCl,(NH4)2SO4) and detergent)
- 15 mM MgCl2
1x PCR solution – 1.5 mM MgCl2
- 2 mM dNTPs of each
1 x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
100 mM MgCl2
Storage conditions: -20ºC
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8ºC has no detrimental effects on the quality of Titan HotTaq 10x PCR Mix.
| ORDERING INFORMATION |
| Product |
Quantity |
Cat. # |
| Titan HotTaq 10x PCR Mix B1 |
1 ml (500 reactions) |
BT10602 |
| Titan HotTaq 10x PCR Mix B2 |
1 ml (500 reactions) |
BT10603 |
Comments: This product is supplied for research use only.
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