Applications:
- cDNA synthesis.
- RNA analysis by primer extension.
- DNA labeling.

Description: The Titan H Minus M-MLV Reverse Transcriptase is a genetically modified M-MLV RT which exhibits RNA or DNA dependent DNA polymerase, but lacks ribonuclease H activity. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of the RNase H activity results in an increase of full-length cDNA products. The enzyme has RNA polymerization-dependent and DNA polymerization-dependent activity but lacks ribonuclease H activity.

Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of  1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37ºC.

Quality control: Free of endo- and exodeoxyribonucleases, phosphatases and ribonuclease. Activity and stability tested in first strand cDNA synthesis.

Storage conditions: 50% glycerol (v/v), 50 mM Tris-HCl pH 8.0 at 25ºC, 100 mM NaCl, 5 mM DTT, 1 mM EDTA, 0.1% NP-40. Store at -20°C.

Concentration: 200 units/µl

Supplied with:
5 x RT Reaction Buffer 1 (with MgCl₂ and DTT) 0.25 M Tris-HCl, 0.5 M KCl, 30 mM MgCl₂, 25 mM DTT
5 x RT Reaction Buffer 2 (Mg²⁺ and DTT free) 0.25 M Tris-HCl, 0.5 M KCl
25 mM MgCl₂
20 mM MnCl₂
100 mM DTT

Comments: This product is supplied for research use only.