Applications:
- Suited for a wide range of PCR assays.
- Primer extension.
- TA cloning.

Source: Purified from an E. coli strain carrying an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.

Description: Titan Taq DNA Polymerase (Figure 1) has 5’ to 3’ polymerisation-dependent exonuclease replacement activity but lacks 3’ to 5’ exonuclease activity. The enzyme has “extendase activity” allowing TA cloning.

Figure 1. Amplification of 420 bp target DNA using Titan Taq DNA Polymerase.
M – Atlas Star 100 bp DNA Ladder
1-3 – Titan Taq DNA Polymerase

Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 min at 74ºC.

Quality control: The enzyme is free of nicking and priming activities, exonucleases and unspecific endonucleases. SDS/PAGE - 95 kD band, >98% pure. Activity and stability tested via thermocycling. The error rate per nucleotide per cycle is ~2.5 x 10^-5; the accuracy is ~ 4 x 10⁴. Estimated half life at 95°C is 1.5 hours.

Storage & Dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Concentration: 5 units/µl

Supplied with:
10 x Reaction Buffer 1 (Mg²⁺ free): 800 mM Tris-HCl pH 9.4 at 25°C, 200 mM (NH₄)₂SO₄, 0.2% w/v Tween-20.
10 x Reaction Buffer 2 (Mg²⁺ and detergent free): 800 mM Tris-HCl pH 9.4 at 25°C, 200 mM (NH₄)₂SO₄.
25 mM MgCl₂
10x Enhancer 1
Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution).
Enhancer 1 is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.

Shipping & Storage conditions: Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this reagent. However, routine storage at -20°C is strongly recommended.

Comments: This product is supplied for research use only.

To place an order, please fill in our order form, and email to info@bioatlas.com or fax +372 616 6755.