Applications:
- Primer extension.
- MALDI-TOF mass spectrometry.
- Mass-array.

Source: Purified from an E. coli strain carrying an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.

Description: The enzyme has 5’ to 3’ polymerisation-dependent exonuclease replacement activity ONLY and enhanced efficiency for incorporating unconventional nucleotides (labeled ddNTPs & acyclo nucleotides).

Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 min at 74°C.

Quality control: Titan Thermo DNA Polymerase is free of nicking and priming activities, exonucleases and unspecific endonucleases. Activity and stability tested via thermocycling. Estimated half life at 95°C: >60 minutes. Error rate per nucleotide per cycle: ~8.0x10^-5.

Storage & Dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Concentration: 5 units/µl

Supplied with:
10x Reaction Buffer Thermo: 500 mM Tris-HCl pH 9.5
100 mM MgCl₂

In some applications 0.5x Buffer final concentration is recommended.
0.5x Buffer final concentration is 25 mM Tris-HCl and 5 mM MgCl₂.

Shipping & Storage conditions: Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this reagent. However, routine storage at -20°C is strongly recommended.

Comments: This product is supplied for research use only.