Applications:
- Detection and quantification of DNA and cDNA targets.
- Profiling gene expression.
- Microbial detection.
- Viral load determination.

Description: Titan HotTaq Probe qPCR Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and Titan HotTaq DNA polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

Titan HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Titan HotTaq Probe qPCR Mix is available with ROX or no ROX dye.

Cycler and Probe compatibility:
Titan HotTaq Probe qPCR Mix (ROX) is compatible for use with any probe system and qPCR cyclers requiring high ROX dye level, including ABI PRISM® 5700, 7000, 7300, 7700, 7900 and 7900HT (including Fast-Block); Stratagene Mx3000P™, Mx3005P™ and Mx4000^®.

Titan HotTaq Probe qPCR Mix (no ROX) is compatible for use with any probe system and qPCR cyclers that do not require reference dye, including Bio-Rad iQ™ 5, Opticon™, Opticon™ 2; Chromo 4™, MiniOpticon, CFX96, CFX384; Cepheid SmartCycler™ ; Corbett Rotor-Gene™ 3000, Rotor-Gene™ 6000; Eppendorf Mastercycler^® ep realplex; Roche LightCycler^® 480 and Techne Quantica™.

Storage conditions: Routine storage: -20ºC. Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of Titan HotTaq Probe qPCR Mix.

Composition:
Titan HotTaq DNA polymerase
5x qPCR buffer P
15 mM MgCl₂: 1 x PCR solution – 3 mM MgCl₂
dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP
ROX dye*
or no ROX dye

*ROX dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.

Comments: This product is supplied for research use only.