Atlas LongTaq DNA Polymerase


APPLICATIONS:

– Standard and long PCR.

– PCR with difficult templates.


DESCRIPTION:

Atlas LongTaq DNA Polymerase is the mixture of two DNA polymerases designed for long PCR having the 3´ to 5´ proofreading exonuclease activity (Fig.1). The enzyme allows to amplify genomic DNA fragments up to 20 kb and viral DNA fragments up to 40 kb.

Figure 1. Amplification of 5,3 Kb target DNA using Atlas LongTaq DNA Polymerase.
1- 2
– Atlas LongTaq DNA Polymerase
M – Atlas Star 1 Kb DNA Ladder Plus

Concentration: 5 unit/µl

Unit definition: One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.

Source: Recombinant E. coli strain with cloned gene encoding Thermus aquaticus DNA polymerase.

Quality control: Free of detectable, non-specific nucleases.

Supplied with:
Atlas 10x Taq Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% Nonident P40.
Atlas 10x Taq Buffer with (NH4)2SO4: 750 mM Tris-HCl (pH 8.8 at 25°C),
200 mM (NH4)2SO4, 0.1% Tween 20.
Atlas 25 mM MgCl2

Storage conditions: 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol. Store at -20°C. Guaranteed stable for 12 months when properly stored.

Comments: This product is supplied for research use only.

ORDERING INFORMATION
Product Quantity Cat. # Data Sheet MSDS
Atlas LongTaq DNA Polymerase 500 units BA00403
Atlas LongTaq DNA Polymerase 1000 units BA00404
Atlas LongTaq DNA Polymerase 2500 units BA00405