Atlas Taq DNA Polymerase


APPLICATIONS:

– Polymerase chain reaction (PCR).

– Primer extension.

– DNA sequencing.

 

DESCRIPTION:

Atlas Taq DNA Polymerase is highly thermostable DNA polymerase (Fig.1). The enzyme catalyzes 5’ to 3’ synthesis of DNA, but lacks 3’ to 5’ exonuclease activity.


Figure 1. Amplification of 420 bp target DNA using Atlas Taq DNA Polymerase.
M – Atlas Star 100 bp DNA Ladder
1-3 – Atlas Taq DNA Polymerase

Concentration: 5 units/µl

Unit definition: One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.

Source: Recombinant E. coli strain with cloned gene encoding Thermus aquaticus DNA polymerase.

Quality control: Free of detectable, non-specific nucleases.

Supplied with:
Atlas 10x Taq Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% Nonident P40.
Atlas 10x Taq Buffer with (NH4)2SO4: 750 mM Tris-HCl (pH 8.8 at 25°C),
200 mM (NH4)2SO4, 0.1% Tween 20.
Atlas 25 mM MgCl2

Storage conditions: 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol. Store at -20°C. Guaranteed stable for 12 months when properly stored.

Comments: This product is supplied for research use only.


ORDERING INFORMATION
Product Quantity Cat. # Data Sheet MSDS
Atlas Taq DNA Polymerase 500 units BA00103
Atlas Taq DNA Polymerase 1000 units BA00104
Atlas Taq DNA Polymerase 2500 units BA00105