Titan H Minus M-MLV Reverse Transcriptase


APPLICATIONS:

– cDNA synthesis.

– RNA analysis by primer extension.

– DNA labeling.


DESCRIPTION:

The Titan H Minus M-MLV Reverse Transcriptase is a genetically modified M-MLV RT which exhibits RNA or DNA dependent DNA polymerase, but lacks ribonuclease H activity. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of the RNase H activity results in an increase of full-length cDNA products. The enzyme has RNA polymerization-dependent and DNA polymerization-dependent activity but lacks ribonuclease H activity.

Concentration: 200 units/µl

Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of  1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37ºC.

Quality control: Free of endo- and exodeoxyribonucleases, phosphatases and ribonuclease. Activity and stability tested in first strand cDNA synthesis.

Storage conditions: 50% glycerol (v/v), 50 mM Tris-HCl pH 8.0 at 25ºC, 100 mM NaCl, 5 mM DTT, 1 mM EDTA, 0.1% NP-40. Store at -20°C.

Supplied with:
5 x RT Reaction Buffer 1 (with MgCl2 and DTT) 0.25 M Tris-HCl, 0.5 M KCl, 30 mM MgCl2, 25 mM DTT
5 x RT Reaction Buffer 2 (Mg2+ and DTT free) 0.25 M Tris-HCl, 0.5 M KCl
25 mM MgCl2
20 mM MnCl2
100 mM DTT

Comments: This product is supplied for research use only

ORDERING INFORMATION
Product Quantity Cat. # Data Sheet MSDS
Titan H Minus M-MLV Reverse Transcriptase 10000 units BT10901