Titan HotTaq EvaGreen® qPCR Capillary Mix

 

APPLICATIONS:

  • Detection and quantification of DNA and cDNA targets.
  • Profiling gene expression.
  • Microbial detection.
  • Viral load determination.

DESCRIPTION:

Titan HotTaq EvaGreen® qPCR Capillary Mix is optimized for real-time quantitative PCR assays for qPCR cyclers with capillaries. The mix includes Titan HotTaq DNA polymerase, dNTPs, MgCl2 and EvaGreen® dye. Only template, primers and water needs to be added.

Titan HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C, preventing the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Cycler compatibility:

Titan HotTaq EvaGreen® qPCR Capillary Mix can be used with LightCycler® 1.x and LightCycler® 2.0 (Roche Applied Sciences).

Composition
Titan HotTaq DNA polymerase
5x qPCR Buffer E
12.5 mM MgCl2: 1 x PCR solution – 2.5 mM MgCl2
dNTPs, including dTTP to improve reaction sensitivity and efficiency
EvaGreen® dye*
BSA

* EvaGreen® is a DNA-binding dye for qPCR that, compared to SYBR® Green I, has similar spectra but much less PCR inhibition. It is extremely stable and nonmutagenic/ noncytotoxic. EvaGreen® is compatible with all common qPCR cyclers by selecting the standard settings for SYBR® Green or FAM.

“EvaGreen® is a registered trademark of BIOTIUM, INC.”

The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer, where such research does not include testing, analysis or screening services for any third party in return for compensation on a per test basis. The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes.

“SYBR® is a registered trademark of Molecular Probes, Inc.”

Storage conditions: Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of Titan HotTaq EvaGreen® qPCR Capillary Mix

Comments: This product is supplied for research use only.

ORDERING INFORMATION
Product Quantity Cat. # Data Sheet MSDS
Titan HotTaq EvaGreen® qPCR Capillary Mix 1 mL (250 reactions) BT11103