Titan HotTaq EvaGreen® qPCR Mix
- Detection and quantification of DNA and cDNA targets.
- Profiling gene expression.
- Microbial detection.
- Viral load determination.
Titan HotTaq EvaGreen® qPCR Mix is optimized for real-time quantitative PCR assays. The mix includes Titan HotTaq DNA polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye (and ROX dye according to system requirements). Only template primers and water need to be added.
Titan HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Titan HotTaq EvaGreen® qPCR Mix is available with ROX or no ROX dye.
Titan HotTaq EvaGreen® qPCR Mix (ROX) can be used with all major qPCR cyclers requiring high ROX dye level, including ABI PRISM® 5700, 7000, 7300, 7700, 7900 and 7900HT (including Fast-Block); Stratagene Mx3000P™, Mx3005P™ and Mx4000®.
Titan HotTaq EvaGreen® qPCR Mix (no ROX) can be used with all major qPCR cyclers that do not require reference dye, including Bio-Rad iQ™ 5, Opticon™, Opticon™ 2; Chromo 4™, MiniOpticon, CFX96, CFX384; Corbett Rotor-Gene™ 3000, Rotor-Gene™ 6000; Roche LightCycler® 480 and Techne Quantica™.
Titan HotTaq DNA polymerase
5x qPCR buffer E
12.5 mM MgCl2: 1 x PCR solution – 2.5 mM MgCl2
dNTPs, including dTTP to improve reaction sensitivity and efficiency
or no ROX dye
Storage conditions: Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of Titan HotTaq EvaGreen® qPCR Mix.
* EvaGreen® is a DNA-binding dye for qPCR that, compared to SYBR® Green I, has similar spectra but much less PCR inhibition. It is extremely stable and nonmutagenic/ noncytotoxic. EvaGreen® is compatible with all common qPCR cyclers by selecting the standard settings for SYBR® Green or FAM.
“EvaGreen® is a registered trademark of BIOTIUM, INC.”
The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer, where such research does not include testing, analysis or screening services for any third party in return for compensation on a per test basis. The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes.
“SYBR® is a registered trademark of Molecular Probes, Inc.”
**ROX dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.
Comments: This product is supplied for research use only.
|Product||Quantity||Cat. #||Data Sheet||MSDS|
|Titan HotTaq EvaGreen® qPCR Mix (ROX)||1 mL (250 reactions)||BT11101|
|Titan HotTaq EvaGreen® qPCR Mix (no ROX)||1 mL (250 reactions)||BT11102|