Titan HotTaq EvaGreen® HRM Mix
APPLICATIONS:
- High Resolution Melt (HRM)
DESCRIPTION:
Titan HotTaq EvaGreen® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: Titan HotTaq DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. Only water, template and primers need to be added.
Titan HotTaq DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Titan HotTaq EvaGreen® HRM Mix is available with ROX or no ROX dye.
Composition:
- Titan HotTaq DNA polymerase
- 5 x EvaGreen®HRM buffer
- dNTPs
- 5 mM MgCl2 1 x PCR solution – 2.5 mM MgCl2
- EvaGreen® dye*
- BSA
- ROX dye**
or no ROX dye
Storage conditions:
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of Titan HotTaq EvaGreen® HRM Mix.
* EvaGreen® is a DNA-binding dye for qPCR that, compared to SYBR® Green I, has similar spectra but much less PCR inhibition. It is extremely stable and nonmutagenic/ noncytotoxic. EvaGreen® is compatible with all common qPCR cyclers by selecting the standard settings for SYBR® Green or FAM.
“EvaGreen® is a registered trademark of BIOTIUM, INC.”
**ROX dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.
| ORDERING INFORMATION | ||||
| Product | Quantity | Cat. # | Data Sheet | MSDS |
| Titan HotTaq EvaGreen® HRM Mix (ROX) | 1 mL (250 rxn) | BT11201 |  |
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| Titan HotTaq EvaGreen® HRM Mix (no ROX) | 1 mL (250 rxn) | BT11202 | |
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