Titan HotTaq EvaGreen® HRM Mix



  • High Resolution Melt (HRM)


Titan HotTaq EvaGreen® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: Titan HotTaq DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. Only water, template and primers need to be added.

Titan HotTaq DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Titan HotTaq EvaGreen® HRM Mix is available with ROX or no ROX dye.


  • Titan HotTaq DNA polymerase
  • 5 x EvaGreen®HRM buffer
  • dNTPs
  • 5 mM MgCl2 1 x PCR solution – 2.5 mM MgCl2
  • EvaGreen® dye*
  • BSA
  • ROX dye**
    or no ROX dye

Storage conditions:

Routine storage: -20ºC

Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of Titan HotTaq EvaGreen® HRM Mix.

* EvaGreen® is a DNA-binding dye for qPCR that, compared to SYBR® Green I, has similar spectra but much less PCR inhibition. It is extremely stable and nonmutagenic/ noncytotoxic. EvaGreen® is compatible with all common qPCR cyclers by selecting the standard settings for SYBR® Green or FAM.
“EvaGreen® is a registered trademark of BIOTIUM, INC.”

**ROX dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.

Product Quantity Cat. # Data Sheet MSDS
Titan HotTaq EvaGreen® HRM Mix (ROX) 1 mL (250 rxn) BT11201
Titan HotTaq EvaGreen® HRM Mix (no ROX) 1 mL (250 rxn) BT11202