Titan HotTaq EvaGreen® qPCR Universal Mix

APPLICATIONS:

• Detection and quantification of DNA and cDNA targets
• Profiling gene expression
• Microbial detection
• Viral load determination

Advantages:

• Specific and reproducible real time PCR
• Highly efficient in case of low copy number targets
• Superior performance with long (up to 500 bp) and GC-rich templates
• UNG treatment capability due to combination of dUTP/dTTP
• Easy pipetting due to blue visualisation dye

DESCRIPTION:

Titan HotTaq EvaGreen® qPCR Universal Mix is optimized for real-time quantitative PCR assays. The ready-to-use mix includes Titan HotTaq DNA polymerase and other components necessary to perform the reaction – only water, template and primers needs to be added.

Titan HotTaq DNA polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Composition:

• Titan HotTaq DNA polymerase
• qPCR buffer
• 5 mM MgCl₂: 1x PCR solution – 2.5 mM MgCl₂
• dNTPs, including dUTP/dTTP
• EvaGreen® dyeInternal reference (based on ROX dye)
• GC-enhancer
• Visualisation dye

Shipping and Storage conditions:

Routine storage: -20ºC

Shipping at room temperature has no detrimental effects on the quality of this reagent.

ORDERING INFORMATION
Product	Quantity	Cat. #	Data Sheet	MSDS
Titan HotTaq EvaGreen® qPCR Universal Mix	1 mL (250 rxn)	BT11104