Titan HotTaq EvaGreen® qPCR Universal Mix



  • Detection and quantification of DNA and cDNA targets
  • Profiling gene expression
  • Microbial detection
  • Viral load determination


  • Specific and reproducible real time PCR
  • Highly efficient in case of low copy number targets
  • Superior performance with long (up to 500 bp) and GC-rich templates
  • UNG treatment capability due to combination of dUTP/dTTP
  • Easy pipetting due to blue visualisation dye


Titan HotTaq EvaGreen® qPCR Universal Mix is optimized for real-time quantitative PCR assays. The ready-to-use mix includes Titan HotTaq DNA polymerase and other components necessary to perform the reaction – only water, template and primers needs to be added.

Titan HotTaq DNA polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.


  • Titan HotTaq DNA polymerase
  • qPCR buffer
  • 5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
  • dNTPs, including dUTP/dTTP
  • EvaGreen® dyeInternal reference (based on ROX dye)
  • GC-enhancer
  • Visualisation dye

Shipping and Storage conditions:

Routine storage: -20ºC

Shipping at room temperature has no detrimental effects on the quality of this reagent.

Product Quantity Cat. # Data Sheet MSDS
Titan HotTaq EvaGreen® qPCR Universal Mix 1 mL (250 rxn) BT11104