Titan HotTaq Probe qPCR Capillary Mix

 

APPLICATIONS:

  • Detection and quantification of DNA and cDNA targets.
  • Profiling gene expression.
  • Microbial detection.
  • Viral load determination.

DESCRIPTION:

Titan HotTaq Probe qPCR Capillary Mix is optimized for real-time quantitative PCR assays for qPCR cyclers with capillaries. The mix contains several components necessary to perform qPCR, including Titan HotTaq DNA polymerase. Only template, primers, probe and water needs to be added.
Titan HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C, preventing the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Cycler compatibility:
Titan HotTaq Probe qPCR Capillary Mix can be used with LightCycler® 1.x and LightCycler® 2.0 (Roche Applied Sciences).

Composition
Titan HotTaq DNA polymerase
5x qPCR Buffer P
15 mM MgCl2: 1 x PCR solution – 3 mM MgCl2
dNTPs, including dTTP to improve reaction sensitivity and efficiency
BSA

Storage conditions: Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of Titan HotTaq Probe qPCR Capillary Mix.

Comments: This product is supplied for research use only.

ORDERING INFORMATION
Product Quantity Cat. # Data Sheet MSDS
Titan HotTaq Probe qPCR Capillary Mix 1 mL (250 reactions) BT11003