Atlas H Minus M-MLV Reverse Transcriptase

APPLICATIONS:

– cDNA synthesis

– RNA analysis by primer extension

– DNA labeling

DESCRIPTION:

Atlas H Minus M-MLV Reverse Transcriptase is a genetically modified M-MLV RT which exhibits RNA or DNA dependent DNA polymerase, but lacks ribonuclease H activity. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of the RNase H activity results in an increase of full-length cDNA products. The enzyme has RNA polymerization-dependent and DNA polymerization-dependent activity but lacks ribonuclease H activity.

Reagents Provided:

• Atlas H Minus M-MLV Reverse Transcriptase
• 5x RT Reaction Buffer(with added DTT) – 250 mM Tris-HCl (pH8.3), 375 mM KCl, 15 mM MgCl₂, 50 mM DTT.
• 5 x RT Reaction Buffer (DTT free) – 250 mM Tris-HCl (pH8,3), 375 mM KCl, 15 mM MgCl₂
• 100 mM DTT

Shipping and Storage conditions:

Routine storage: -20ºC

Shipping at room temperature has no detrimental effects on the quality of this reagent.

ORDERING INFORMATION
Product	Quantity	Cat. #	Data Sheet	MSDS
Atlas H Minus M-MLV Reverse Transcriptase	10 000 U (200 U/µl)	BA10902